Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
2.
Haplotype and phylogenetic analysis of OLR1(Intron
I) gene in jaffarabadi and surti buffalo -
Nadeem Shabir, C V Jawale, C D Bhong, Mehrajuddin
Naikoo, D N Rank, C G Joshi
Vet World. 2011; 4(9): 396-398
doi: 10.5455/vetworld.2011.396-398
Abstract
The present study was carried out to reveal
haplotype and phylogenetic analysis of OLR1 gene
in Jaffarabadi and Surti breeds of buffalo. Twenty
nucleotide sequences generated from our previous
study (Shabir, 2009) were used to reveal the
haplotypes in OLR1 (Intron I) gene in the
population of Jaffarabadi and Surti buffalo. Four
haplotypes viz. H1 (CTA), H2 (TCT), H3 (CTT) and
H4 (TTT) were observed with frequencies of 0.05,
0.1, 0.15 and 0.7 respectively in the population
studied. Phylogenetic analysis of these twenty
sequences with the same region of Bos taurus
distributed the sequences into four clusters based
on the homology between them. Cluster I contained
two sequences ( jb3 and jb8) bearing a common SNP
at nucleotide position 843. Cluster III contained
s3, s4 and s7 since they possessed a common SNP at
nucleotide position 423, however s3 remained as an
out group in this cluster since it contained an
additional SNP at position 866. The remaining
sequences had highest homology and fell in cluster
II while as the sequence of Bos taurus remained as
an out group on account of the difference in bases
at many loci.
Keywords: Phylogenetics,
haplotypes, OLR1 and Bubalus bubalis.
